Journal: OncoTargets and therapy

Article Title: miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

doi: 10.2147/OTT.S103460

Figure Lengend Snippet: NRP2 is a target of miR-486-5p. Notes: ( A ) Effect of miR-486-5p on NRP2 expression as determined by a luciferase reporter assay. The data were normalized by determining the firefly and Renilla luciferase activities measured at 72 hours posttransfection; SW620 and HT-29 cells were transfected with miR-486-5p mimic (miR-486-5p group), mimic control (NC group), and blank control (blank group). ( B ) Transfection of miR-486-5p mimics to SW620 and HT-29 cells increases the expression of miR-486-5p detected by qRT-PCR. ( C ) NRP2 protein expression level was significantly decreased in miR-486-5p-transfected SW620 and HT-29 cells. ( D ) Relative NRP2 protein levels in figure ( C ) were quantified using the gray scale quantification analysis. ** P <0.01. Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NRP2, neuropilin-2; qRT-PCR, quantitative real-time polymerase chain reaction; NC, nontarget control.

Article Snippet: Human CRC cell lines SW620 and HT-29 were purchased from Boster Company (Boster, Wuhan, People’s Republic of China).

Techniques: Expressing, Luciferase, Reporter Assay, Transfection, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

doi: 10.2147/OTT.S103460

Figure Lengend Snippet: miR-486-5p inhibited xenograft tumor growth by targeting NRP2 in vivo. Notes: ( A ) Groups of nude mice were implanted subcutaneously with SW620/PBS cells (Blank group), SW620/mimic-control cells (NC group), or SW620/miR-486-5p cells (miR-486-5p group), Tumor volumes were recorded at the indicated times. ( B ) The tumor mass was determined when the mice were sacrificed. ( C ) NRP2 expression in tumors was examined by Western blot. ( D ) Quantitative analysis of the NRP2 protein level using a gray scan analysis. One representative tumor mass group from three independent experiments with similar results is shown. * P <0.05, ** P <0.01. Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NRP2, neuropilin-2; PBS, phosphate-buffered saline; NC, nontarget control.

Article Snippet: Human CRC cell lines SW620 and HT-29 were purchased from Boster Company (Boster, Wuhan, People’s Republic of China).

Techniques: In Vivo, Expressing, Western Blot

Journal: BMC Clinical Pathology

Article Title: Detection of collagen triple helix repeat containing-1 and nuclear factor (erythroid-derived 2)-like 3 in colorectal cancer

doi: 10.1186/1472-6890-12-2

Figure Lengend Snippet: Detection of CTHRC1 and NFE2L3 in Western Blot . A : Recombinant- and native CTHRC1 analysed in total protein extracted from HT29, and SW620 with MAb CH21D7. B, C & D : Recombinant- and native NFE2L3 analysed with PAb anti-NFE2L3 ( B ) or MAb 41HF8 ( C ) in total protein extracted from HT29, SW620, leukocytes and lymphocytes ( D )

Article Snippet: The human colon adenocarcinoma cell lines HT29 and SW620 were kindly supplied by Oryzon Genomics (Barcelona, Spain), originally acquired from the American Tissue Culture Collection (ATCC)(Manassas, VA, USA).

Techniques: Western Blot, Recombinant

Journal: BMC Clinical Pathology

Article Title: Detection of collagen triple helix repeat containing-1 and nuclear factor (erythroid-derived 2)-like 3 in colorectal cancer

doi: 10.1186/1472-6890-12-2

Figure Lengend Snippet: Summary of the analysis of CTHRC1 and NFE2L3 by Western blot

Article Snippet: The human colon adenocarcinoma cell lines HT29 and SW620 were kindly supplied by Oryzon Genomics (Barcelona, Spain), originally acquired from the American Tissue Culture Collection (ATCC)(Manassas, VA, USA).

Techniques: Western Blot

Journal: BMC Clinical Pathology

Article Title: Detection of collagen triple helix repeat containing-1 and nuclear factor (erythroid-derived 2)-like 3 in colorectal cancer

doi: 10.1186/1472-6890-12-2

Figure Lengend Snippet: CTHRC1 detection by DAS-ELISA . A : Detection of the recombinant CTHRC1- and negative control protein (NFE2L3). B : Detection of the native CTHRC1 protein in colon cell lines HT29 and SW620, and E. coli protein extract used as negative control. The MAb CH21D7 was used as captured antibody and biotin-conjugated CH24G2 as detection antibody. The cellular extracts or control antigens were incubated in the wells during 1 h at 24°C or overnight at 4°C.

Article Snippet: The human colon adenocarcinoma cell lines HT29 and SW620 were kindly supplied by Oryzon Genomics (Barcelona, Spain), originally acquired from the American Tissue Culture Collection (ATCC)(Manassas, VA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Negative Control, Control, Incubation

Journal: BMC Clinical Pathology

Article Title: Detection of collagen triple helix repeat containing-1 and nuclear factor (erythroid-derived 2)-like 3 in colorectal cancer

doi: 10.1186/1472-6890-12-2

Figure Lengend Snippet: NFE2L3 detection by DAS-ELISA . A & B: Detection of the recombinant NFE2L3- and negative control protein (CTHRC1) and analysis of NFE2L3 in protein extracts from HT29, SW620, leukocytes, and E. coli protein extract with the antibodies pair PAb α-NFE2L3 and MAb 41HF8. C & D: Detection of the recombinant NFE2L3- and negative control protein (CTHRC1) and analysis of NFE2L3 in protein extracts from HT29, SW620, leukocytes, and E. coli protein extract with the pair sFab E5 and MAb 41HF8. The samples were incubated during 1 h at 24°C or overnight at 4°C.

Article Snippet: The human colon adenocarcinoma cell lines HT29 and SW620 were kindly supplied by Oryzon Genomics (Barcelona, Spain), originally acquired from the American Tissue Culture Collection (ATCC)(Manassas, VA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Negative Control, Incubation